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| Registros recuperados : 310 | |
| 14. |  | IGUMA, L. T.; SÁ, W. F. de; VIANA, J. H. M.; CAMARGO, L. S. de A. Avances tecnólogicos en la reproducción animal: inseminación artificial. In: MARTINS, P. do C.; DINIZ, F. H.; MOREIRA, M. S. de P.; NOGUEIRA NETTO, V.; ARCURI, P. B. (Ed.). Conocimientos y estratégias tecnológicas para la producción de leche en regiones tropicales. Juiz de Fora: Embrapa Gado de Leite, 2007. p. 309-319.| Biblioteca(s): Embrapa Gado de Leite. |
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| 15. | | VIANA, J. H. M.; IGUMA, L. T.; CAMARGO, L. S. de A.; SÁ, W. F. de. Avances tecnológicos en la reproducción animal: superovulación y transferencia de embriones. In: MARTINS, P. do C.; DINIZ, F. H.; MOREIRA, M. S. de P.; NOGUEIRA NETTO, V.; ARCURI, P. B. (Ed.). Conocimientos y estratégias tecnológicas para la producción de leche en regiones tropicales. Juiz de Fora: Embrapa Gado de Leite, 2007. p. 321-332.| Biblioteca(s): Embrapa Gado de Leite. |
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| 16. | | CAMARGO, L. S. de A.; OLIVEIRA, C. S.; PFEIFER, L. F. M.; MACHADO, R. Avanços e inovações em biotécnicas da reprodução em bovinos de leite. In: VILELA, D.; FERREIRA, R. de P.; FERNANDES, E. N.; JUNTOLLI, F. V. (Ed.). Pecuária de leite no Brasil: cenários e avanços tecnológicos. Brasília, DF: Embrapa, 2016. p. 325-343.| Biblioteca(s): Embrapa Gado de Leite; Embrapa Pecuária Sudeste. |
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Registro Completo
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
26/12/2018 |
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Data da última atualização: |
24/01/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 4 |
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Autoria: |
OTERO, R.; HERNÁNDEZ, D.; CAMARGO, L. S. de A. |
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Afiliação: |
LUIZ SERGIO DE ALMEIDA CAMARGO, CNPGL. |
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Título: |
Effect of Trichostatin-A on embryos of bovine clones modified genetically with GFP. |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
Indian Journal of Science and Technology, v. 11, n. 25, 2018. |
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Páginas: |
9 p. |
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DOI: |
10.17485/ijst%2F2018%2Fv11i25%2F128251 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract Objective: To evaluate the effect of treatment with trichostatin-A (TSA) on the production of bovine embryos, expressing the gene of the green fluorescent protein (GFP) generated by SCNT. Materials: 164 oocytes were distributed in three treatments, NT-GFP: newly reconstructed zygotes with genetically modified cells and not subject to TSA. NT-Trico-GFP: newly reconstructed zygotes with genetically modified cells and subjected to TSA. PART: Zygotes generated by parthenogenetic activation, used as a control for the process of oocyte activation and culture of embryos. The rates of cleavage, blastocysts, and embryos that expressed GFP were assessed by contingency tables and chi-square tests. Results: The percentage of cleavage in the zygotes in the NT-GFP treatment was greater but did not vary significantly from the NT-Trico-GFP treatment. However, this last treatment had a higher percentage of blastocyst formation (p = 0.077). The percentage of blastocysts from cleaved zygotes, the produced embryos were significantly higher (p < 0.05) for the NT-Trico-GFP treatment than for the NT-GFP. In both treatments, all the blastocysts generated expressed the GFP protein. Conclusions:TSA improves the embryonic development of clones of genetically modified cattle that express GFP. |
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Palavras-Chave: |
Embryonic Development; Epigenetic Modification; Nuclear Transfer. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/189328/1/Artigo-Indian-J-Sci-and-Tech-Camargo-Effect.pdf
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Marc: |
LEADER 01943naa a2200205 a 4500
001 2102530
005 2023-01-24
008 2018 bl uuuu u00u1 u #d
024 7 $a10.17485/ijst%2F2018%2Fv11i25%2F128251$2DOI
100 1 $aOTERO, R.
245 $aEffect of Trichostatin-A on embryos of bovine clones modified genetically with GFP.$h[electronic resource]
260 $c2018
300 $a9 p.
520 $aAbstract Objective: To evaluate the effect of treatment with trichostatin-A (TSA) on the production of bovine embryos, expressing the gene of the green fluorescent protein (GFP) generated by SCNT. Materials: 164 oocytes were distributed in three treatments, NT-GFP: newly reconstructed zygotes with genetically modified cells and not subject to TSA. NT-Trico-GFP: newly reconstructed zygotes with genetically modified cells and subjected to TSA. PART: Zygotes generated by parthenogenetic activation, used as a control for the process of oocyte activation and culture of embryos. The rates of cleavage, blastocysts, and embryos that expressed GFP were assessed by contingency tables and chi-square tests. Results: The percentage of cleavage in the zygotes in the NT-GFP treatment was greater but did not vary significantly from the NT-Trico-GFP treatment. However, this last treatment had a higher percentage of blastocyst formation (p = 0.077). The percentage of blastocysts from cleaved zygotes, the produced embryos were significantly higher (p < 0.05) for the NT-Trico-GFP treatment than for the NT-GFP. In both treatments, all the blastocysts generated expressed the GFP protein. Conclusions:TSA improves the embryonic development of clones of genetically modified cattle that express GFP.
653 $aEmbryonic Development
653 $aEpigenetic Modification
653 $aNuclear Transfer
700 1 $aHERNÁNDEZ, D.
700 1 $aCAMARGO, L. S. de A.
773 $tIndian Journal of Science and Technology$gv. 11, n. 25, 2018.
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Embrapa Gado de Leite (CNPGL) |
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